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Applications

Collagen is the most abundant protein in a human body. It is a critical component of almost all organs and tissues, providing the framework for cell attachment and growth. Collagen degradation regulates normal tissue remodeling which occurs during development, homeostasis and repair,^[1] whereas excessive collagen degradation is a key feature of tissues in a variety of diseases, such as cancer, inflammation, and fibrosis.^[2]

All types of collagen from all species share the triple helical protein structure, which is nearly exclusively found in collagen. After being cleaved by a collagenase, the collagen molecule becomes thermally unstable at body temperature and the triple helix spontaneously denatures.^[3,4,5]It is also found that unfolding of the collagen triple helix occurs during mechanical injuries of connective tissues and is an important damage mechanism.^[6]

The founders of 3Helix invented the Collagen Hybridizing Peptide (CHP) that can specifically bind to unfolded collagen molecules, by forming the triple helix with the denatured collagen chains, in a fashion analogous to a primer binding to a melted DNA strand during PCR.^[4] Conjugated with a fluorescent or biotin label, CHP is the first probe of its kind to enable direct detection of unfolded collagen molecules in virtually any tissue that has been subject to mechanical damage,^[6] or enzymatic remodeling (whether physiologically regulated, or disease associated).^[5]

Collagen is also one of the most widely used natural scaffold materials for regenerative medicine. The process of harvesting native extracellular matrices by removing cells from animal tissues (i.e., tissue decellularization) may alter the collagen structure and negatively affect the mechanical property and regenerative capacity of the ECM materials. CHP enables assessment of the structural integrity of collagen matrices from the molecular level, and facilitates optimization of the decellularization protocols.^[7]

Additionally, CHP can be used in several biochemical assays, such as in-gel Western blot,^[8] for identification and possibly quantification of collagen content in a biological sample.

CHPs compared to traditional staining methods

CHP versus conventional collagen characterization methods

Reference

Matrix metalloproteinases and the regulation of tissue remodeling. Nature Reviews Molecular Cell Biology (2007) 8, 221-233.
Targeting and mimicking collagens via triple helical peptide assembly. Current Opinion in Chemical Biology (2013) more info
Collagenase unwinds triple-helical collagen prior to peptide bond hydrolysis. EMBO Journal (2004) 23, 3020-3030.
Targeting collagen strands by photo-triggered triple-helix hybridization. Proceedings of the National Academy of Science of the United States of America (2012) more info
In situ imaging of tissue remodeling with collagen hybridizing peptides. ACS Nano (2017) (open access) more info
Molecular level detection and localization of mechanical damage in collagen enabled by collagen hybridizing peptides. Nature Communications (2017) (open access) more info
Molecular assessment of collagen denaturation in decellularized tissues using a collagen hybridizing peptide. Acta Biomaterialia (2017) (open access) more info
Direct detection of collagenous proteins by fluorescently labeled collagen mimetic peptides. Bioconjugate Chemistry (2013) more info

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Unless we expressly state otherwise in writing, 3HELIX gives no rights to use Products in any non-research commercial application, including manufacturing, quality control, or non-research commercial services such as reporting the results of your non-research activities for a fee or other consideration. If the Customer wishes to use a 3HELIX Product for any non-research commercial purposes, the Customer will require an additional license from 3HELIX by contacting info@3helix.com. 10.3 Intellectual Property Ownership. We exclusively own all intellectual property rights in any inventions (patentable or otherwise), discoveries, improvements, data, know-how, or other results that are conceived, developed, discovered, reduced to practice, or generated by you alone, by or for us, or jointly by you and us, in relation to processes, methods, or related synthesis of a custom product, or otherwise in connection with designing or manufacturing a custom product. 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Any notice or communication required or permitted under these Terms must be in writing and will be deemed received when personally delivered, or 3 business days after being sent by certified mail, postage prepaid, to a party’s specified address. 12.8 Requirement to Reduce to Writing. No waiver, consent, modification, amendment or changes to the terms of this Agreement will be binding unless in writing and signed by both of us. Our failure to object to terms contained in any subsequent communication from you will not be a waiver or modification of our Agreement. Updated Jan 16, 2018

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F-CHP | Collagen Hybridizing Peptide, 5-FAM Conjugate

R-CHP | Collagen Hybridizing Peptide, Cy3 Conjugate

B-CHP | Collagen Hybridizing Peptide, Biotin Conjugate

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F-CHP | Collagen Hybridizing Peptide, 5-FAM Conjugate

R-CHP | Collagen Hybridizing Peptide, Cy3 Conjugate

B-CHP | Collagen Hybridizing Peptide, Biotin Conjugate

Applications

Popular Products

F-CHP | Collagen Hybridizing Peptide, 5-FAM Conjugate

R-CHP | Collagen Hybridizing Peptide, Cy3 Conjugate

B-CHP | Collagen Hybridizing Peptide, Biotin Conjugate

Popular Products

F-CHP | Collagen Hybridizing Peptide, 5-FAM Conjugate

R-CHP | Collagen Hybridizing Peptide, Cy3 Conjugate

B-CHP | Collagen Hybridizing Peptide, Biotin Conjugate

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