Frequently Asked Questions
- Technical Questions
- General Questions & Logistics
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For more detailed information about using CHPs please view our User Guide
All CHP peptides are 30 amino acids in length.
F-CHP is labeled with fluorescein for direct fluorescence detection.
R-CHP is labeled with sulfo-Cyanine 3 for direct fluorescence detection.
B-CHP is labeled with biotin for avidin/streptavidin-mediated detection. B-CHP offers flexible detection options, such as non-green fluorescence and HRP-enzyme methods, to avoid background noise and enhance signal.
In vivo CHP is labeled with sulfo-Cynanine 7.5 incorporates our newest sequence which allows them to be directly injected into animals without having to pre-activate them with a heating step.
Auto-CHP is made for use in auto-staining platforms and is available for select partners.
Collagen hybridizing peptides are proven to specifically recognize denatured collagen molecules in a variety of tissues, species, and disease models.
CHPs are especially stable, however the attached dyes are not as stable once reconstituted.
CHPs can be stored in -20°C as a powder for long term storage and 4°C after reconstitution in water.
For F-CHP and B-CHP, there is no need to aliquot and freeze. For the R-CHP and the in vivo CHP, they should be used immediately or aliquoted to reduce the freeze-thaw cycles.
R-CHP, F-CHP, and the in vivo CHP must be protected from light.
For fluorescent imaging (e.g., when using F-CHP or R-CHP), we recommend using formaldehyde, instead of paraformaldehyde or glutaraldehyde.
After years of working with F-CHP and B-CHP, we found no limit of its heating/cooling cycles. The peptide is very stable both chemically and physically. However, we do NOT recommend users to heat up the stock solution. The recommended procedure is that a user only aliquot out the needed amount from the stock solution (e.g., 10 µL, 50 µM) and dilute it to the required concentration.*not applicable for in vivo CHP, see User Guide
When co-staining samples with CHP and antibodies, we recommend blocking the tissue slides with 10% serum or 5% BSA. (For certain tissue types, e.g., kidney, it may be necessary to block endogenous biotin using a standard kit for B-CHP staining.)
General Questions & Logistics
CHPs are very stable and can be shipped without temperature controls, however we recommend that you store the peptide in -20° for long term storage as a powder.
You will receive an email with tracking information once your order has been processed and shipped.
If you work in a University, Hospital, Research Facility, or business that uses Purchase Orders we also accept payments on a Net 30 basis. Please email your purchase orders to email@example.com. You may also select 'Purchase Order' as your method of payment when checking out on our site.
3Helix stands behind the quality of our products and will work with you in the event of a quality control issue. Please feel free to contact us via email firstname.lastname@example.org in order to resolve any issues you may have with your order.