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Collagen Hybridizing Peptide, Biotin Conjugate (B-CHP), Product Vial

Collagen Hybridizing Peptide, Biotin Conjugate (B-CHP)

SKU: BIO60
Availability: In Stock
$218.00 USD
$218.00 USD
Unit: 60μg

Specifications

Use case: Validation
Ideal for: New users

Purchase Orders Accepted at Checkout

User Guides with FAQ
User Guides with FAQ
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Description
Description
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Description

The collagen hybridizing peptide (CHP) is a novel and unique peptide that specifically binds unfolded collagen chains, both in vitro and in vivo.[1,2,3] By sharing the Gly-X-Y repeating sequence of natural collagen, CHP has a strong capability to hybridize with denatured collagen chains by reforming the triple helical structure, in a fashion similar to DNA fragments annealing to complementary DNA strands. CHP is extremely specific: it has negligible affinity to intact collagen molecules due to lack of binding sites, and it is inert towards non-specific binding because of its neutral and hydrophilic nature.

CHP is a powerful histopathology tool which enables straightforward detection of inflammation and tissue damage caused by a large variety of diseases, as well as tissue remodeling during development and aging.[3] CHP robustly visualizes the pericellular matrix turnover caused by proteolytic migration of cancer cells within 3D collagen culture, without the use of synthetic fluorogenic matrices or genetically modified cells.[4] CHP can measure and localize mechanical injury to collagenous tissue at the molecular level.[5] It also enables assessment of collagen denaturation in decellularized extracellular matrix.[6] In addition, CHP can be used to specifically visualize collagen bands in SDS-PAGE gels without the need for western blot.[7]

B-CHP is labeled with biotin for avidin/streptavidin-mediated detection. B-CHP offers flexible detection options, such as non-green fluorescence and HRP-enzyme methods, to avoid background and enhance signal.

Specificity: CHP binds to the unfolded triple-helical chains of all collagen types (e.g., I, II, III, IV, etc).[3,7]

Applications: immunofluorescence,[3] immunohistochemistry[3]

Specification

Synonyms B-CHP, collagen mimetic peptide (CMP)
Molecular weight 2762.01 g/mol
Purity 85% by HPLC
Conjugate Single biotin tag per peptide
Content Purified lyophilized powder
Storage -20 °C as powder, 4 °C after reconstitution in water
Shipping  Ambient temperature

 

Features

  • More informative, reliable and convenient than zymography, DQ collagen, SHG, and TEM
  • High affinity and unparalleled specificity to collagen with essentially no nonspecific binding
  • Applicable to all types of collagen from all species, relying on collagen's secondary structure instead of any defined sequence for binding
  • Suitable for both frozen and paraffin-embedded sections with no need for antigen retrieval
  • A non-antibody approach with no species restrictions against any co-staining antibody
  • Small size (2% of IgG by MW) enabling facile tissue penetration and whole specimen staining without sectioning
  • Stable in solution under 4 °C, eliminating the need to aliquot for storage

 

Special applications

B-CHP provides the flexibility for visualizing damaged collagen using any user-selected fluorophore (e.g., AlexaFluor dyes conjugated streptavidin), or a non-fluorescence method (e.g., through NeutrAvidin-HRP mediated DAB staining).

In addition, B-CHP and commercial streptavidin-conjugated gold nanoparticles can be used in combination to label unfolded collagen molecules under TEM: collagen fibers in an intact or mechanically overloaded rat tail tendon probed by B-CHP and streptavidin conjugated 10nm-sized gold nanoparticles (scale bar: 200 nm).


For research use only. Not intended or approved for diagnostic or therapeutic use.

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Shipping & Returns
Shipping & Returns
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Order Processing


Please allow 1-2 Business Days to process your order. Expect an email within 2 Business Days to let you know your order is on the way!

LOCAL PICKUP - 675 S Arapeen Drive, Salt Lake City, UT 84108

Orders selected for local pickup can be picked up Monday- Friday, from 9am-4pm. An email will be sent when your order is ready for pickup.

FLAT RATE SHIPPING DOMESTIC & INTERNATIONAL

Orders are generally shipped within 1-2 Business Days using FedEx 2 Day or FedEx International Priority. Flat rate shipping fee of $39 applies to orders shipping to USA. Flat rate shipping fee of $77 applies to orders shipping WORDLWIDE. You may also opt to ‘Use Your Own FedEx Account’ at checkout and shipping will be billed directly to your account.

REFUNDS, RETURNS, AND EXCHANGES

We do not accept returns of any kind.

In the event that your order arrives damaged in any way, please email us as soon as possible at orders@3helix.com with your order number and a photo of the item’s condition. We address these on a case-by-case basis but will try our best to work towards a satisfactory solution.

If you have any further questions, please don't hesitate to contact us at orders@3helix.com.
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Citations (37)
Citations (37)
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  • Perticarari, S., et al. (2024). A Novel Multiplex Staining Method for Simultaneous Detection of Multiple Antigens in Formalin-Fixed Paraffin-Embedded Tissues. Journal of Histochemistry & Cytochemistry, 72(2), 79–94. doi: 10.1369/00221554231225995
  • Kim, M. J., et al. (2024). Advances in Understanding the Pathogenesis of Abdominal Aortic Aneurysm: Focus on Extracellular Matrix Remodeling. Biomedicines, 12(1), 230. doi: 10.3390/biomedicines12010230
  • Caves, E. A. (2024). Mechanisms and Functions of Lipodystrophy in Dermal Fibrosis [Doctoral dissertation, Yale University]. ProQuest Dissertations & Theses Global. Link to dissertation (ProQuest)
  • Nakagawa, K., & Nakashima, Y. (2024). Pathogenesis of human atheroma necrotic core: degradation of connective tissue fibers and possible involvement of cathepsin K. Translational Medicine Communications, 9, 26. doi: 10.1186/s41231-024-00188-6
  • Brown, A., et al. (2023). Natural Retinol Analogs Potentiate the Effects of Retinal on Aged and Photodamaged Skin: Results from In Vitro to Clinical Studies. Dermatology and Therapy, 13(10), 2299–2317. doi: 10.1007/s13555-023-01004-z
  • Petrocelli, J. J., et al. (2023). Disuse-induced muscle fibrosis, cellular senescence, and senescence-associated secretory phenotype in older adults are alleviated during re-ambulation with metformin pre-treatment. Aging Cell, 22(11), e13936. doi: 10.1111/acel.13936
  • Gallate, Z. S., et al. (2023). Galectin-3 and RAGE differentially control advanced glycation endproduct-induced collagen damage in murine intervertebral disc organ culture. JOR Spine, 6(2), e1254. doi: 10.1002/jsp2.1254
  • Kim, M., et al. (2023). Recent Advances in Collagen-Based Biomaterials for Bone Tissue Engineering. Polymers (Basel), 15(18), 3683. doi: 10.3390/polym15183683
  • Schiele, M. A., et al. (2023). Effect of altered collagen cross-linking on the multiscale mechanics and damage behavior of the rat Achilles tendon. Journal of Biomechanics, 151, 111546. doi: 10.1016/j.jbiomech.2023.111546
  • Feng, X., et al. (2023). Apical expansion of calvarial osteoblasts and suture patency is dependent on graded fibronectin cues. bioRxiv. Preprint. doi: 10.1101/2023.01.16.524278
  • Lee, S. J., et al. (2023). A midposition NOTCH3 truncation in inherited cerebral small vessel disease may affect the protein interactome. The Journal of Biological Chemistry, 299(1), 102772. doi: 10.1016/j.jbc.2022.102772
  • Sherman, M. A., et al. (2022). Collagen metabolism alterations in the tumor microenvironment and cancer progression. Aging Cancer, 3(2), 116–129. doi: 10.1002/aac2.12049
  • Jenkins, E. C., et al. (2022). Age alters the oncogenic trajectory toward luminal mammary tumors that activate unfolded proteins responses. Aging Cell, 21(10), e13665. doi: 10.1111/acel.13665
  • Gallate, Z. S. (2022). Galectin-3 Mediates Advanced Glycation Endproduct-Induced Collagen Damage in Intervertebral Discs [Master's thesis, Icahn School of Medicine at Mount Sinai]. Icahn School of Medicine at Mount Sinai Academic Works. Link to thesis
  • Luce, A. L., et al. (2022). Role of periostin in cardiac fibrosis and dysfunction. American Journal of Physiology-Heart and Circulatory Physiology, 323(1), H165–H175. doi: 10.1152/ajpheart.00148.2022
  • Hwang, J., et al. (2022). Noninvasive label-free deep-tissue imaging of collagen fiber remodeling in the cornea using polarization-sensitive optical coherence tomography. Biomedical Optics Express, 13(4), 2346–2363. doi: 10.1364/BOE.448286
  • Li, Y., et al. (2022). Engineering collagen-targeting peptides for improved diagnosis and therapy of fibrotic diseases. International Journal of Molecular Sciences, 23(8), 4245. doi: 10.3390/ijms23084245
  • Hettinger, Z. R., et al. (2022). Mechanotherapy Reprograms Aged Muscle Stromal Cells to Remodel the Extracellular Matrix during Recovery from Disuse. Function (Oxford, England), 3(3), zqac015. doi: 10.1093/function/zqac015
  • Viiklepp, K., et al. (2022). C1r Upregulates Production of Matrix Metalloproteinase-13 and Promotes Invasion of Cutaneous Squamous Cell Carcinoma. The Journal of Investigative Dermatology, 142(5), 1478–1488.e9. doi: 10.1016/j.jid.2021.10.008
  • Jussila, A. R., et al. (2022). Skin Fibrosis and Recovery Is Dependent on Wnt Activation via DPP4. The Journal of Investigative Dermatology, 142(6), 1597–1606.e9. doi: 10.1016/j.jid.2021.10.025
  • Rognoni, E., et al. (2021). Role of distinct fibroblast lineages and immune cells in dermal repair following UV radiation-induced tissue damage. eLife, 10, e71052. doi: 10.7554/eLife.71052
  • Zhang, X., et al. (2021). Hydrolysis of a second Asp-Pro site at the N-terminus of NOTCH3 in inherited vascular dementia. Scientific Reports, 11(1), 17246. doi: 10.1038/s41598-021-96679-9
Collagen Hybridizing Peptide, Biotin Conjugate (B-CHP), Product Vial

Collagen Hybridizing Peptide, Biotin Conjugate (B-CHP)

$218.00
Collagen Hybridizing Peptide, Biotin Conjugate (B-CHP), Product Vial
Collagen Hybridizing Peptide, Biotin Conjugate (B-CHP)
Collagen Hybridizing Peptide, Biotin Conjugate (B-CHP)
Collagen Hybridizing Peptide, Biotin Conjugate (B-CHP)

Collagen Hybridizing Peptide, Biotin Conjugate (B-CHP)

$218.00
Unit: 60μg

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