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Collagen Hybridizing Peptide, Cy3 Conjugate (R-CHP), Product Vial

Collagen Hybridizing Peptide, Cy3 Conjugate (R-CHP)

SKU: RED300
Availability: In Stock
$605.00 USD
$605.00 USD
Unit: 300µg

Specifications

Use case: Full studies
Ideal for: Publishing

Purchase Orders Accepted at Checkout

User Guides with FAQ
User Guides with FAQ
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Description
Description
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Description

The collagen hybridizing peptide (CHP) is a novel and unique peptide that specifically binds unfolded collagen chains, both in vitro and in vivo.[1,2,3] By sharing the Gly-X-Y repeating sequence of natural collagen, CHP has a strong capability to hybridize with denatured collagen chains by reforming the triple helical structure, in a fashion similar to DNA fragments annealing to complementary DNA strands. CHP is extremely specific: it has negligible affinity to intact collagen molecules due to lack of binding sites, and it is inert towards non-specific binding because of its neutral and hydrophilic nature.

CHP is a powerful histopathology tool which enables straightforward detection of inflammation and tissue damage caused by a large variety of diseases, as well as tissue remodeling during development and aging.[3] CHP robustly visualizes the pericellular matrix turnover caused by proteolytic migration of cancer cells within 3D collagen culture, without the use of synthetic fluorogenic matrices or genetically modified cells.[4] CHP can measure and localize mechanical injury to collagenous tissue at the molecular level.[5] It also enables assessment of collagen denaturation in decellularized extracellular matrix.[6] In addition, CHP can be used to specifically visualize collagen bands in SDS-PAGE gels without the need for western blot.[7]

R-CHP is labeled with sulfo-Cyanine3 for direct fluorescence detection.

Specificity: CHP binds to the unfolded triple-helical chains of all collagen types (e.g., I, II, III, IV, etc).[3,7]

Applications: immunofluorescence,[3] cell imaging,[4] SDS-PAGE (in-gel western)[7]

Specification

Synonyms R-CHP, collagen mimetic peptide (CMP)
Molecular weight 3191.44 g/mol
Purity 85% by HPLC
Conjugate Single sulfo-Cyanine3 tag per peptide
Excitation 548 nm
Emission 563 nm
Content Purified lyophilized powder
Storage -20 °C as powder, 4 °C after reconstitution in water
Shipping Ambient Temperature

 

Features

  • More informative, reliable and convenient than zymography, DQ collagen, SHG, and TEM
  • High affinity and unparalleled specificity to collagen with essentially no nonspecific binding
  • Applicable to all types of collagen from all species, relying on collagen's secondary structure instead of any defined sequence for binding
  • Suitable for both frozen and paraffin-embedded sections with no need for antigen retrieval
  • A non-antibody approach with no species restrictions against any co-staining antibody
  • Small size (2% of IgG by MW) enabling facile tissue penetration and whole specimen staining without sectioning
  • Stable in solution under 4 °C, eliminating the need to aliquot for storage 

For research use only. Not intended or approved for diagnostic or therapeutic use.

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Shipping & Returns
Shipping & Returns
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Order Processing


Please allow 1-2 Business Days to process your order. Expect an email within 2 Business Days to let you know your order is on the way!

LOCAL PICKUP - 675 S Arapeen Drive, Salt Lake City, UT 84108

Orders selected for local pickup can be picked up Monday- Friday, from 9am-4pm. An email will be sent when your order is ready for pickup.

FLAT RATE SHIPPING DOMESTIC & INTERNATIONAL

Orders are generally shipped within 1-2 Business Days using FedEx 2 Day or FedEx International Priority. Flat rate shipping fee of $39 applies to orders shipping to USA. Flat rate shipping fee of $77 applies to orders shipping WORDLWIDE. You may also opt to ‘Use Your Own FedEx Account’ at checkout and shipping will be billed directly to your account.

REFUNDS, RETURNS, AND EXCHANGES

We do not accept returns of any kind.

In the event that your order arrives damaged in any way, please email us as soon as possible at orders@3helix.com with your order number and a photo of the item’s condition. We address these on a case-by-case basis but will try our best to work towards a satisfactory solution.

If you have any further questions, please don't hesitate to contact us at orders@3helix.com.
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Selected Citations
Selected Citations
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  • Fei, Y., et al. (2024). Promoting Chondrogenesis by Targeted Delivery to the Degenerating Cartilage in Early Treatment of Osteoarthritis. Bioactive Materials, 40, 624–633. doi: 10.1016/j.bioactmat.2024.08.004
  • Fang, Y., et al. (2024). Collagen Denaturation in Post-Run Achilles Tendons and Achilles Tendinopathy: In Vivo Mechanophysiology and Magnetic Resonance Imaging. Science Advances, 10(40), eado2015. doi: 10.1126/sciadv.ado2015
  • Rappold, R., et al. (2024). Salmonella‐Driven Intestinal Edema in Mice is Characterized by Tensed Fibronectin Fibers. The FEBS Journal. Advance online publication. doi: 10.1111/febs.17190
  • Dhiman, M. S., et al. (2024). Collagen Integrity of the Annulus Fibrosus in Degenerative Disc Disease Individuals Quantified with Collagen Hybridizing Peptide. JOR Spine, 7(3), e1359. doi: 10.1002/jsp2.1359
  • Satoh, A., et al. (2024). Keratinocyte-Driven Dermal Collagen Formation in the Axolotl Skin. Developmental Biology, 509, 56–66. doi: 10.1016/j.ydbio.2024.03.003
  • Zhao, J., et al. (2024). Delineating, Imaging, and Assessing Pulmonary Fibrosis Remodeling via Collagen Hybridization. ACS Nano, 18(41), 27997–28011. doi: 10.1021/acsnano.4c06139
  • Ahmad, A., et al. (2024). Molecular Imaging of Collagen Remodeling in a Murine Model of Lung Fibrosis. bioRxiv. Preprint. doi: 10.1101/2024.03.01.582999
  • Wang, H., et al. (2024). Chiral Arginine Modified Electrospun Membrane for Enhancing Tendon Healing. Advanced Functional Materials, 2402845. doi: 10.1002/adfm.202402845
  • Xiao, Z., et al. (2023). Desmoplastic Stroma Restricts T Cell Extravasation and Mediates Immune Exclusion and Immunosuppression in Solid Tumors. bioRxiv. Preprint. doi: 10.1101/2023.04.13.536777
  • Loflin, B. E., et al. (2023). An Adolescent Murine In Vivo Anterior Cruciate Ligament Overuse Injury Model. The American Journal of Sports Medicine, 51(7), 1721–1732. doi: 10.1177/03635465231165753
  • Smith, K. A., et al. (2023). Collagen Molecular Damage is a Hallmark of Early Atherosclerosis Development. Journal of Cardiovascular Translational Research, 16(2), 463–472. doi: 10.1007/s12265-022-10316-y
  • Salarian, M., et al. (2023). Homeostatic, Non-Canonical Role of Macrophage Elastase in Vascular Integrity. Circulation Research, 132(4), 432–448. doi: 10.1161/CIRCRESAHA.122.322096
  • Flinn, M. A., et al. (2023). Myofibroblast Ccn3 is Regulated by Yap and Wwtr1 and Contributes to Adverse Cardiac Outcomes. Frontiers in Cardiovascular Medicine, 10, 1142612. doi: 10.3389/fcvm.2023.1142612
  • Lim, P. J., et al. (2023). Perturbations in Fatty Acid Metabolism and Collagen Production Infer Pathogenicity of a Novel MBTPS2 Variant in Osteogenesis Imperfecta. Frontiers in Endocrinology, 14, 1195704. doi: 10.3389/fendo.2023.1195704
  • Ho, Y.-C., et al. (2023). PROX1 Inhibits PDGF-B Expression to Prevent Myxomatous Degeneration of Heart Valves. Circulation Research, 133(6), 463–480. doi: 10.1161/CIRCRESAHA.123.323027
  • Nishi, K., et al. (2023). A Thioacetamide-Induced Liver Fibrosis Model for Pre-Clinical Studies in Microminipig. Scientific Reports, 13(1), 14996. doi: 10.1038/s41598-023-42144-8
  • Zhang, Y., et al. (2023). Omentin-1 Induces Mechanically Activated Fibroblasts Lipogenic Differentiation through PKM2/YAP/PPARγ Pathway to Promote Lung Fibrosis Resolution. Cellular and Molecular Life Sciences, 80(10), 308. doi: 10.1007/s00018-023-04961-y
  • Bui, T. V. A., et al. (2023). Core-Shell Droplet-Based Angiogenic Patches for the Treatment of Ischemic Diseases: Ultrafast Processability, Physical Tunability, and Controlled Delivery of an Angiogenic Cocktail. ACS Applied Materials & Interfaces, 15(44), 50693–50707. doi: 10.1021/acsami.3c09062
  • Sun, A. R., et al. (2023). Hybrid Hydrogel-Extracellular Matrix Scaffolds Identify Distinct Ligand and Mechanical Signatures in Cardiac Aging. bioRxiv. Preprint. doi: 10.1101/2023.10.11.561941
  • Jones, B., et al. (2022). Structurally Abnormal Collagen Fibrils in Abdominal Aortic Aneurysm Resist Platelet Adhesion. Journal of Thrombosis and Haemostasis, 20(2), 470–477. doi: 10.1111/jth.15576
  • Smith, K. A. (2022). Evaluation of Hemodynamic Induced Alterations in Extracellular Matrix Organization in Atherogenesis [Doctoral dissertation, The University of Utah]. University of Utah Research Repository. Link to dissertation
  • Atta, G. (2022). The Lymphogenic and Hemangiogenic Factors Affecting the Human Sclera [Doctoral dissertation, Universität zu Köln]. Kölner UniversitätsPublikationsServer. Link to dissertation
  • Kim, M., et al. (2022). A New Murine Liver Fibrosis Model Induced by Polyhexamethylene Guanidine-Phosphate. Biomolecules & Therapeutics, 30(2), 126–133. doi: 10.4062/biomolther.2021.139
Collagen Hybridizing Peptide, Cy3 Conjugate (R-CHP), Product Vial

Collagen Hybridizing Peptide, Cy3 Conjugate (R-CHP)

$605.00
Collagen Hybridizing Peptide, Cy3 Conjugate (R-CHP), Product Vial
Collagen Hybridizing Peptide, Cy3 Conjugate (R-CHP)
Collagen Hybridizing Peptide, Cy3 Conjugate (R-CHP)

Collagen Hybridizing Peptide, Cy3 Conjugate (R-CHP)

$605.00
Unit: 300µg

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