bchp

Collagen Hybridizing Peptide, Biotin Conjugate (B-CHP)

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SKU: BIO300

Product Description

Description

The collagen hybridizing peptide (CHP) is a novel and unique peptide that specifically binds unfolded collagen chains, both in vitro and in vivo.[1,2,3] By sharing the Gly-X-Y repeating sequence of natural collagen, CHP has a strong capability to hybridize with denatured collagen chains by reforming the triple helical structure, in a fashion similar to DNA fragments annealing to complementary DNA strands. CHP is extremely specific: it has negligible affinity to intact collagen molecules due to lack of binding sites, and it is inert towards non-specific binding because of its neutral and hydrophilic nature.

CHP is a powerful histopathology tool which enables straightforward detection of inflammation and tissue damage caused by a large variety of diseases, as well as tissue remodeling during development and aging.[3] CHP can measure and localize mechanical injury to collagenous tissue at the molecular level.[4] It also enables assessment of collagen denaturation in decellularized extracellular matrix.[5] In addition, CHP can be used to specifically visualize collagen bands in SDS-PAGE gels without the need for western blot.[6]

B-CHP is labeled with biotin for avidin/streptavidin-mediated detection. B-CHP offers flexible detection options, such as non-green fluorescence and HRP-enzyme methods, to avoid background and enhance signal.

Applications: immunofluorescence, immunohistochemistry

Specification

Synonyms B-CHP, collagen mimetic peptide (CMP)
Molecular weight 2762.01 g/mol
Purity 95% by HPLC
Conjugate Single biotin tag per peptide
Content Purified lyophilized powder
Storage -20 °C as powder, 4 °C after reconstitution in water

 

Features

  • More informative, reliable and convenient than zymography, DQ collagen, SHG, and TEM
  • High affinity and unparalleled specificity to collagen with essentially no nonspecific binding
  • Applicable to all types of collagen from all species, relying on collagen's secondary structure instead of any defined sequence for binding
  • Suitable for both frozen and paraffin-embedded sections with no need for antigen retrieval
  • A non-antibody approach with no species restrictions against any co-staining antibody
  • Small size (2% of IgG by MW) enabling facile tissue penetration and whole specimen staining without sectioning
  • Stable in solution under 4 °C, eliminating the need to aliquot for storage

 

Special applications

B-CHP provides the flexibility for visualizing damaged collagen using any user-selected fluorophore (e.g., AlexaFluor dyes conjugated streptavidin), or a non-fluorescence method (e.g., through NeutrAvidin-HRP mediated DAB staining).

In addition, B-CHP and commercial streptavidin-conjugated gold nanoparticles can be used in combination to label unfolded collagen molecules under TEM: collagen fibers in an intact or mechanically overloaded rat tail tendon probed by B-CHP and streptavidin conjugated 10nm-sized gold nanoparticles (scale bar: 200 nm).

biotin-special

 

Key Publications

  1. Targeting and mimicking collagens via triple helical peptide assemblies. Curr. Opin. Chem. Biol., 2013. [link]
  2. Targeting collagen strands by photo-triggered triple-helix hybridization. Proc. Natl. Acad. Sci. U.S.A., 2012. [link]
  3. In situ imaging of tissue remodeling with collagen hybridizing peptides. ACS Nano, 2017. [link]
  4. Molecular level detection and localization of mechanical damage in collagen enabled by collagen hybridizing peptides. Nat. Commun., 2017. [link]
  5. Molecular assessment of collagen denaturation in decellularized tissues using the collagen hybridizing peptide. Acta Biomater., 2017. [link]
  6. Direct detection of collagenous proteins by fluorescently labeled collagen mimetic peptides. Bioconjug. Chem., 2013. [link]

 

Additional information

CHP can slowly self-assemble into the triple helical structure in solution during storage. The trimeric CHP requires a simple heating step prior to usage. Please check the protocol for details: CHP_Datasheet.pdf

For research use only. Not intended or approved for diagnostic or therapeutic use.

 

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