Collagen Hybridizing Peptide Stain: B-CHP Histology Stain
Collagen Hybridizing Peptide, Biotin Conjugate (B-CHP), Product Vial
Collagen Hybridizing Peptide Stain: B-CHP Histology Stain
Collagen Hybridizing Peptide Stain: B-CHP Histology Stain
Collagen Hybridizing Peptide, Biotin Conjugate (B-CHP), Product Vial
Collagen Hybridizing Peptide Stain: B-CHP Histology Stain

B-CHP | Collagen Hybridizing Peptide, Biotin Conjugate

SKU: BIO60
Availability: In Stock
Product Type: B-CHP
$218.00 USD
$218.00 USD

Purchase Orders Accepted at Checkout

Subtotal: $218.00
User Guides (3) with FAQ
User Guides (3) with FAQ
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Description
Description
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Description

The collagen hybridizing peptide (CHP) is a novel and unique peptide that specifically binds unfolded collagen chains, both in vitro and in vivo.[1,2,3] By sharing the Gly-X-Y repeating sequence of natural collagen, CHP has a strong capability to hybridize with denatured collagen chains by reforming the triple helical structure, in a fashion similar to DNA fragments annealing to complementary DNA strands. CHP is extremely specific: it has negligible affinity to intact collagen molecules due to lack of binding sites, and it is inert towards non-specific binding because of its neutral and hydrophilic nature.

CHP is a powerful histopathology tool which enables straightforward detection of inflammation and tissue damage caused by a large variety of diseases, as well as tissue remodeling during development and aging.[3] CHP robustly visualizes the pericellular matrix turnover caused by proteolytic migration of cancer cells within 3D collagen culture, without the use of synthetic fluorogenic matrices or genetically modified cells.[4] CHP can measure and localize mechanical injury to collagenous tissue at the molecular level.[5] It also enables assessment of collagen denaturation in decellularized extracellular matrix.[6] In addition, CHP can be used to specifically visualize collagen bands in SDS-PAGE gels without the need for western blot.[7]

B-CHP is labeled with biotin for avidin/streptavidin-mediated detection. B-CHP offers flexible detection options, such as non-green fluorescence and HRP-enzyme methods, to avoid background and enhance signal.

Specificity: CHP binds to the unfolded triple-helical chains of all collagen types (e.g., I, II, III, IV, etc).[3,7]

Applications: immunofluorescence,[3] immunohistochemistry[3]

Specification

Synonyms B-CHP, collagen mimetic peptide (CMP)
Molecular weight 2762.01 g/mol
Purity 95% by HPLC
Conjugate Single biotin tag per peptide
Content Purified lyophilized powder
Storage -20 °C as powder, 4 °C after reconstitution in water

 

Features

  • More informative, reliable and convenient than zymography, DQ collagen, SHG, and TEM
  • High affinity and unparalleled specificity to collagen with essentially no nonspecific binding
  • Applicable to all types of collagen from all species, relying on collagen's secondary structure instead of any defined sequence for binding
  • Suitable for both frozen and paraffin-embedded sections with no need for antigen retrieval
  • A non-antibody approach with no species restrictions against any co-staining antibody
  • Small size (2% of IgG by MW) enabling facile tissue penetration and whole specimen staining without sectioning
  • Stable in solution under 4 °C, eliminating the need to aliquot for storage

 

Special applications

B-CHP provides the flexibility for visualizing damaged collagen using any user-selected fluorophore (e.g., AlexaFluor dyes conjugated streptavidin), or a non-fluorescence method (e.g., through NeutrAvidin-HRP mediated DAB staining).

In addition, B-CHP and commercial streptavidin-conjugated gold nanoparticles can be used in combination to label unfolded collagen molecules under TEM: collagen fibers in an intact or mechanically overloaded rat tail tendon probed by B-CHP and streptavidin conjugated 10nm-sized gold nanoparticles (scale bar: 200 nm).

 

For research use only. Not intended or approved for diagnostic or therapeutic use.

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Shipping & Returns
Shipping & Returns
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Order Processing


Please allow 1-2 Business Days to process your order. Expect an email within 2 Business Days to let you know your order is on the way!

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REFUNDS, RETURNS, AND EXCHANGES

We do not accept returns of any kind.

In the event that your order arrives damaged in any way, please email us as soon as possible at orders@3helix.com with your order number and a photo of the item’s condition. We address these on a case-by-case basis but will try our best to work towards a satisfactory solution.

If you have any further questions, please don't hesitate to contact us at orders@3helix.com.
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Citations (37)
Citations (37)
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  • J Histochem Cytochem. 2024 Feb;72(2):79-94. doi: 10.1369/00221554231225995
  • Biomedicines. 2024 Jan 19;12(1):230. doi: 10.3390/biomedicines12010230
  • Caves, Elizabeth Ann. Mechanisms and Functions of Lipodystrophy in Dermal Fibrosis. Diss. Yale University, 2024.
  • Nakagawa, K., Nakashima, Y. Pathogenesis of human atheroma necrotic core: degradation of connective tissue fibers and possible involvement of cathepsin K. transl med commun 9, 26 (2024). https://doi.org/10.1186/s41231-024-00188-6
  • Brown, Anthony et al. “Natural Retinol Analogs Potentiate the Effects of Retinal on Aged and Photodamaged Skin: Results from In Vitro to Clinical Studies.” Dermatology and therapy vol. 13,10 (2023): 2299-2317. doi:10.1007/s13555-023-01004-z
  • Petrocelli, Jonathan J et al. “Disuse-induced muscle fibrosis, cellular senescence, and senescence-associated secretory phenotype in older adults are alleviated during re-ambulation with metformin pre-treatment.” Aging cell vol. 22,11 (2023): e13936. doi:10.1111/acel.13936
  • Gallate, Zachary S et al. “Galectin-3 and RAGE differentially control advanced glycation endproduct-induced collagen damage in murine intervertebral disc organ culture.” JOR spine vol. 6,2 e1254. 28 Mar. 2023, doi:10.1002/jsp2.1254
  • Polymers (Basel). 2023 Sep 7;15(18):3683. doi: 10.3390/polym15183683
  • J Biomech. 2023 Apr;151:111546. doi: 10.1016/j.jbiomech.2023.111546
  • Feng, Xiaotian et al. “Apical expansion of calvarial osteoblasts and suture patency is dependent on graded fibronectin cues.” bioRxiv : the preprint server for biology 2023.01.16.524278. 16 Jan. 2023, doi:10.1101/2023.01.16.524278. Preprint.
  • Lee, Soo Jung et al. “A midposition NOTCH3 truncation in inherited cerebral small vessel disease may affect the protein interactome.” The Journal of biological chemistry vol. 299,1 (2023): 102772. doi:10.1016/j.jbc.2022.102772
  • Aging Cancer. 2022 Jun;3(2):116-129. doi: 10.1002/aac2.12049. Epub 2022 Apr 19.
  • Jenkins, Edmund Charles et al. “Age alters the oncogenic trajectory toward luminal mammary tumors that activate unfolded proteins responses.” Aging cell vol. 21,10 (2022): e13665. doi:10.1111/acel.13665
  • Gallate, Zachary S. Galectin-3 Mediates Advanced Glycation Endproduct-Induced Collagen Damage in Intervertebral Discs. MS thesis. Icahn School of Medicine at Mount Sinai, 2022.
  • Am J Physiol Heart Circ Physiol. 2022 Jul 1;323(1):H165-H175. doi: 10.1152/ajpheart.00148.2022. Epub 2022 Jun 3.
  • Biomed Opt Express. 2022 Mar 22;13(4):2346-2363. doi: 10.1364/BOE.448286. eCollection 2022 Apr 1.
  • Int J Mol Sci. 2022 Apr 12;23(8):4245. doi: 10.3390/ijms23084245
  • Hettinger, Zachary R et al. “Mechanotherapy Reprograms Aged Muscle Stromal Cells to Remodel the Extracellular Matrix during Recovery from Disuse.” Function (Oxford, England) vol. 3,3 zqac015. 24 Mar. 2022, doi:10.1093/function/zqac015
  • Viiklepp, Kristina et al. “C1r Upregulates Production of Matrix Metalloproteinase-13 and Promotes Invasion of Cutaneous Squamous Cell Carcinoma.” The Journal of investigative dermatology vol. 142,5 (2022): 1478-1488.e9. doi:10.1016/j.jid.2021.10.008
  • Jussila, Anna R et al. “Skin Fibrosis and Recovery Is Dependent on Wnt Activation via DPP4.” The Journal of investigative dermatology vol. 142,6 (2022): 1597-1606.e9. doi:10.1016/j.jid.2021.10.025
  • Rognoni, Emanuel et al. “Role of distinct fibroblast lineages and immune cells in dermal repair following UV radiation-induced tissue damage.” eLife vol. 10 e71052. 23 Dec. 2021, doi:10.7554/eLife.71052
  • Zhang, Xiaojie et al. “Hydrolysis of a second Asp-Pro site at the N-terminus of NOTCH3 in inherited vascular dementia.” Scientific reports vol. 11,1 17246. 26 Aug. 2021, doi:10.1038/s41598-021-96679-9
  • Filiberti,
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Collagen Hybridizing Peptide Stain: B-CHP Histology Stain

B-CHP | Collagen Hybridizing Peptide, Biotin Conjugate

$218.00
Collagen Hybridizing Peptide Stain: B-CHP Histology Stain
Collagen Hybridizing Peptide, Biotin Conjugate (B-CHP), Product Vial
Collagen Hybridizing Peptide Stain: B-CHP Histology Stain

B-CHP | Collagen Hybridizing Peptide, Biotin Conjugate

$218.00
Unit: 60μg

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