3Helix | CHPs vs Picrosirius Red: Superior Collagen Staining for Accur – 3Helix Inc

Achieve more accurate collagen analysis with CHPs

Picrosirius Red (PSR) staining has long been used to visualize total collagen, but its non-specific binding and reliance on polarized light create challenges in reproducibility and quantitative analysis. In contrast, Collagen Hybridizing Peptides (CHPs) selectively bind to denatured collagen, offering a molecularly precise alternative.

Compared to Picrosirius Red, Collagen Hybridizing Peptides enable:

Superior Sensitivity: Detects lower collagen levels and subtle changes missed by PSR
Less Background: Clearer images with reduced nonspecific binding
Accurate Quantification: More precise and reliable collagen measurement
Greater Versatility: Works with more imaging techniques and experimental setups

CHPs reveal a clearer collagen signal compared to PSR for improved targeting.

Comparative analysis shows how CHPs are:

Designed for exclusive collagen binding, eliminating non-specific staining seen with PSR.
Compatible with automated staining platforms for more efficient high-throughput research
Highly reproducible, eliminating the intra-observer subjectivity of PSR stains.

See the Data

Enhanced Collagen Visualization: Beyond Traditional Staining

While outperforming traditional total collagen stains, CHPs are also the best measurement tool for inflammation and tissue damage across numerous conditions.

Intensity of BCHP staining correlates with disease activity in MASH mouse model

At 3Helix, our CHP products provide:

Compatibility with most dyes, imaging probes, and automated staining platforms
Utility in histology, in vivo studies, and 3D cell cultures
Flexibility for use across any animal model and in human studies
The only product on the market to stain damaged collagen with applications available in total collagen staining

Our CHP products are conjugated with a variety of detection motifs for brightfield and fluorescence detection, and they can be customized to meet your unique needs. Visit our products page to learn more.

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Want to learn how CHPs can help you surpass your research goals?

Our experts are here to help.

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1. Contract Terms 1.1 General Terms. These terms and conditions (“Terms”) and our quotation (if any) comprise the agreement (“Agreement”) between you and 3HELIX Inc, in which case such agreement applies, you agree to accept and be bound by the Agreement by ordering products on 3helix.com or if you receive ordering or sales documents that reference these Terms. This agreement is the complete and exclusive contract between us with repect to your purchase of the products.1.2 Term Conflict. If any conditions within the Agreement documents conflict with each other, we will give them the following priority: the quotation, and then these Terms. We expressly reject any different terms or provisions contained in any document you provide, and if the terms and conditions in this Agreement differ from the terms of your offer, this Agreement will serve as the governing terms for our contract.1.3 When Agreement Takes Effect. 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Unless we expressly state otherwise in writing, 3HELIX gives no rights to use Products in any non-research commercial application, including manufacturing, quality control, or non-research commercial services such as reporting the results of your non-research activities for a fee or other consideration. If the Customer wishes to use a 3HELIX Product for any non-research commercial purposes, the Customer will require an additional license from 3HELIX by contacting info@3helix.com. 10.3 Intellectual Property Ownership. We exclusively own all intellectual property rights in any inventions (patentable or otherwise), discoveries, improvements, data, know-how, or other results that are conceived, developed, discovered, reduced to practice, or generated by you alone, by or for us, or jointly by you and us, in relation to processes, methods, or related synthesis of a custom product, or otherwise in connection with designing or manufacturing a custom product. 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You must comply with the EAR and all other applicable laws, regulations, laws, treaties, and agreements relating to the export, re-export, and import of any Item. You must not, directly or indirectly, without first obtaining the required license to do so from the appropriate U.S. government agency; (a) export, re-export, distribute or supply any Item to (a) any restricted or embargoed country or to a person or entity whose privilege to participate in exports has been denied or restricted by the U.S. government; (b) any person or entity who is involved in improper development or use of nuclear weapons or of chemicals/biological weapons, or missiles, or in terrorist activities. You will, if we request, provide information on the end user and end use of any Item you export or plan to export. 11.4 Audit Cooperation. 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Any notice or communication required or permitted under these Terms must be in writing and will be deemed received when personally delivered, or 3 business days after being sent by certified mail, postage prepaid, to a party’s specified address. 12.8 Requirement to Reduce to Writing. No waiver, consent, modification, amendment or changes to the terms of this Agreement will be binding unless in writing and signed by both of us. Our failure to object to terms contained in any subsequent communication from you will not be a waiver or modification of our Agreement. Updated Jan 16, 2018

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