A great new paper from Dr. Yang Li's group published in ACS NAno utilized in vivo CHPs with a Cy5 fluorescent tag to evaluate collagen destruction in the spine. This in-depth work identified collagen damage in three different animal models, compared the in vivo CHP imaging to micro-CT, histopathology, and performed tissue clearing methods to image using light-sheet fluorescence microscopy to truly identify the anatomic localization of CHP binding in the ...
It seems that CHPs are making an impact for researchers study intervertebral disc degeneration! Another interesting paper published in JCI Insight focused on intervertebral disc (and cartilage) degeneration. Researchers looked at the role of the actin-branching Arp2/3 complex has in maintaining the health of the IVD and cartilage through the regulation of cell-ECM interactions. In this study, they showed that the mutant mice (Arp2/3 deficient mice) had ...
A new article published in the Journal of the Mechanical Behavior of Biomedical Materials used F-CHPs (Figure 7) to assess the damage in an intervertebral disc (IVD) model. Researchers used CHPs to help validate their new mouse disc organ culture system by dynamically applying compression loading in a customized micro-culture device tailored for mouse lumbar discs. They tested native, static, and loaded discs to see how mechanical damage affects disc he...
Newly published article from Dr.'s Xudong Li and Yong Wang utilized F-CHPs to validate a new microfluidic disc-on-a-chip platform for studying disc degeneration. In this work, they used CHPs to quantify the amount of denatured collagen in their new platform and compared it to native tissue, and static tissue culture platform. CHPs showed that their new platform more closely resembled the native tissue, while the static design resulted in higher levels ...
Hot off the press! Exciting new work from Dr. Xudong Li's Lab at the University of Virginia established a comprehensive and detailed quantification method of intervertebral discs in aging and inflammatory conditions. This is the first time anyone demonstrated a linear correlation between natural aging and denatured collagen content in mouse discs, and they saw a robust correlation with conventional histology techniques as well. The image below shows ho...
Newly published work from the Illien-Jünger Lab ( Department of Orthopaedics, Icahn School of Medicine at Mount Sinai) utilized CHPs as a molecular level probe to assess the damage to collagen in intervertebral disc degeneration (IVD) based on a diet that contained high or low levels of advanced glycation end-products (AGEs). These contribute to diabetes and obesity which are associated with back pain. Their results show there is a distinct difference ...
