Dr. Yong-Su Jin and team used CHPs in their research to follow collagen synthesis and degradation through an IPF (idiopathic pulmonary fibrosis) model using bleomycin and the resolution of fibrosis thereafter. Because biochemical assays (the golden standard) show consistently high levels of hydroxyproline content, it fails to detect that fibrosis can resolve itself. Jin et al. used CHPs and Sirius red in histopathology to observe that collagen l...
A great new paper from Dr. Yang Li's group published in ACS NAno utilized in vivo CHPs with a Cy5 fluorescent tag to evaluate collagen destruction in the spine. This in-depth work identified collagen damage in three different animal models, compared the in vivo CHP imaging to micro-CT, histopathology, and performed tissue clearing methods to image using light-sheet fluorescence microscopy to truly identify the anatomic localization of CHP binding in the ...
We are excited to announce that 3Helix has rolled out a new CHP for histology! We now offer R-CHP, which has a sulfo-Cyanine 3 conjugated onto it. The R-CHP has an excitation/emission of 548/563 nm which will fluoresce in the red channel. It still has high specificity for denatured collagen and follows the same protocol as the F-CHP. Now you can image denatured collagen in the red (R-CHP) or green (F-CHP) channels on your favorite fluorescence microsco...
Our founders and inventors of the CHP for 3Helix, Dr.'s Yang Li and Michael Yu, published a chapter titled "In Situ Detection of Degraded and Denatured Collagen via Triple Helical Hybridization: New Tool in Histopathology" in Collagen!! They are thankful for the co-editors and the tremendous contributions of all authors and experts of the field that made this volume possible. They hope that this book on collagen will be a useful tool for scientists wor...
