A new paper published in Circulation Research used CHPs as a way to study the effects of fibroblast-specific Smad3 loss on collagen fragmentation and denaturation. They found that the FS3KO (fibroblast-specific Smad3 knockout) mice showed much higher collagen degradation (Bottom) than the Smad3 fl/fl (flox/flox) mice (top). They saw that smad3 activated cardiac fibroblasts play an important protective role, preserving the ECM network, suppressing macro...
We at 3Helix want to help researchers solve problems in their field and obtain new insights with our CHP products. We are working on commercializing a NEW fluorescently labeled CHP for in vivo use! The new probe will provide highly specific binding to areas of collagen turnover in animal models. This new probe has a unique sequence which interferes with their inherent ability to self-trimerize, making it easy and reliable to use in vivo. This work was ...
Brand new work published in Developmental Cell by Stephen Weiss and our founder Michael Yu! They evaluated protease-dependent invasion of breast cancer cells by examining how ECM remodeling and branching morphogenesis are influenced by specific matrix metalloproteinases (MMPs). They utilized CHPs to analyze the collagen proteolysis in remodeling events along developing mammary epithelial ducts.
Abstract: Metastasizing breast carcinoma cells have been h...
Another exciting article that was published by the Watt Lab (King's College) in PLoS One utilized CHP technology to examine differences in dermal development in mice caused by lysyl oxidase-like 2 (LOXL2) which contributes to the remodelling of the ECM. They used the new CHP to assess and quantify the difference in collagen fiber structure between a LOXL2 knock-out, knock-in and control mice from postnatal day 2 up to day 120.
Abstract: Lysyl oxidase-l...
