Enrichment of Collagen Fragments Using Dimeric Collagen Hybridizing Peptide for Urinary Collagenomics

Enrichment of Collagen Fragments Using Dimeric Collagen Hybridizing Peptide for Urinary Collagenomics
A great new paper from our founder's lab, Dr. Michael Yu at the University of Utah! They developed a dimerized version of the CHP that is linked on the C-terminus that allows for faster-refolding kinetics which can be useful for creating ELISA assays to detect low levels of damaged collagen in biological fluids. They show that it has the ability to capture fragments of collagen that would otherwise go unnoticed and were able to facilitate early peptido...

Delayed Fracture Healing in Obesity-associated Type 2 Diabetic Mouse Model

Delayed Fracture Healing in Obesity-associated Type 2 Diabetic Mouse Model
In this interesting article published in Bone, researchers from Penn State utilized CHPs to help monitor fracture healing in the diet-induced obesity (DIO) mouse model. They applied numerous characterization techniques to probe the microstructure of collagen within a wound (fracture) healing environment. They evaluated collagen structure in a healthy (lean) mouse model and compared it with the DIO model using second harmonic generation (SHG), micro-CT, ...

Another great article about intervertebral disc degeneration!!

Another great article about intervertebral disc degeneration!!
It seems that CHPs are making an impact for researchers study intervertebral disc degeneration! Another interesting paper published in JCI Insight focused on intervertebral disc (and cartilage) degeneration. Researchers looked at the role of the actin-branching Arp2/3 complex has in maintaining the health of the IVD and cartilage through the regulation of cell-ECM interactions. In this study, they showed that the mutant mice (Arp2/3 deficient mice) had ...

Raman Spectroscopy: Guiding Light for the Extracellular Matrix

Raman Spectroscopy: Guiding Light for the Extracellular Matrix
 A very interesting review article recently published in Frontiers in Bioengineering and Biotechnology explores how Raman Spectroscopy (RS) can be used to investigate the ECM. They showcase how RS can be helpful in probing the subtle structural changes in the ECM in cartilage, bone, cancer, and collagen assessment. They can distinguish between collagen and other ECM proteins due to the unique triple-helix and the amino acid composition. However, the dis...

The Impact of Skeletal Muscle Contraction on CD146+Lin- Pericytes

The Impact of Skeletal Muscle Contraction on CD146+Lin- Pericytes
Interesting new work published in The American Journal of Physiology- Cell Physiology evaluated the ability of muscle-derived pericyte to contribute to skeletal muscle remodeling in response to training. The researchers used electric stimulation to contract muscles and monitor the response of pericyte cells. They found that the CD146+ Lin- pericyte cells were the most sensitive to contraction and they contribute to upregulating ECM remodeling and angio...

Clumps of Mesenchymal Stem Cell/Extracellular Matrix Complexes Generated with Xeno-Free Conditions Facilitate Bone Regeneration via Direct and Indirect Osteogenesis

Clumps of Mesenchymal Stem Cell/Extracellular Matrix Complexes Generated with Xeno-Free Conditions Facilitate Bone Regeneration via Direct and Indirect Osteogenesis
A recent article published in the International Journal of Molecular Sciences utilized CHPs to verify the health of collagen in their MSC-ECM complexes. The authors developed a clumped-MSC/ECM (C-MSC) complex under serum-free conditions that allows bone regeneration upon implantation without the use of an artificial scaffold. They grew the C-MSC and compared a cellularized vs decellularized C-MSCs for their ability to promote osteogenesis after implanta...

Acoustic modification of collagen hydrogels facilitates cellular remodeling

Acoustic modification of collagen hydrogels facilitates cellular remodeling
A newly published article from Dr. Hocking's Lab used F-CHPs to image collagen remodeling due to acoustic disturbance. They found that exposing the collagen hydrogels to ultrasound waves revealed areas of cell-mediated collagen fiber remodeling. These areas of collagen remodeling were corroborated with SHG imaging. These results indicate that ultrasound exposure does not directly alter triple-helical conformation of collagen, but sensitizes t...

Developing Regenerative Treatments for Developmental Defects, Injuries, and Diseases Using Extracellular Matrix Collagen-Targeting Peptides

Developing Regenerative Treatments for Developmental Defects, Injuries, and Diseases Using Extracellular Matrix Collagen-Targeting Peptides
A great review article was recently published in the International Journal of Molecular Sciences that discusses how currently available collagen targeting methods are used for regenerative therapeutics. This article also compares the benefits and drawbacks of several collagen targeting strategies. (Spoiler: They highlight several key advantages CHPs have over other collagen-binding peptides). Very informative article and useful to those who are looking...

Protective Effects of Activated Myofibroblasts in the Pressure-Overloaded Myocardium Are Mediated Through Smad-Dependent Activation of a Matrix-Preserving Program

Protective Effects of Activated Myofibroblasts in the Pressure-Overloaded Myocardium Are Mediated Through Smad-Dependent Activation of a Matrix-Preserving Program
A new paper published in Circulation Research used CHPs as a way to study the effects of fibroblast-specific Smad3 loss on collagen fragmentation and denaturation. They found that the FS3KO (fibroblast-specific Smad3 knockout) mice showed much higher collagen degradation (Bottom) than the Smad3 fl/fl (flox/flox) mice (top). They saw that smad3 activated cardiac fibroblasts play an important protective role, preserving the ECM network, suppressing macro...

Visualizing collagen proteolysis by peptide hybridization: From 3D cell culture to in vivo imaging

Visualizing collagen proteolysis by peptide hybridization: From 3D cell culture to in vivo imaging
We at 3Helix want to help researchers solve problems in their field and obtain new insights with our CHP products. We are working on commercializing a NEW fluorescently labeled CHP for in vivo use! The new probe will provide highly specific binding to areas of collagen turnover in animal models. This new probe has a unique sequence which interferes with their inherent ability to self-trimerize, making it easy and reliable to use in vivo. This work was ...
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