Effective enzymatic debridement of burn wounds depends on the denaturation status of collagen

Effective enzymatic debridement of burn wounds depends on the denaturation status of collagen
 Another great paper using CHPs! A recent article published in Wound Repair and Regeneration evaluated how the level of collagen denaturation within burned tissues influences the effectiveness of enzymatic debridement (removal of damaged tissue). They showed that low-temperature burns (<65 °C) had inadequate debridement, while high-temperature burns (>65 °C) had very effective debridement. By using CHPs, Sirius red (PSR), and second harmonic gener...

Malaria parasite immune evasion and adaptation to its mosquito host is influenced by the acquisition of multiple blood meals

Malaria parasite immune evasion and adaptation to its mosquito host is influenced by the acquisition of multiple blood meals
Another great article using CHPs! In this interesting paper, coming out of Dr. Ryan C. Smith's lab from Iowa State, they utilized CHPs to examine the damage done in the midgut of mosquitoes after blood or protein meal feeding. They go on to say that this damage from multiple feedings impacts malaria oocytes' survival. They found that human malaria parasites captured host resources to facilitate their growth after multiple feedings when the basal lamina ...

Tendon tissue microdamage and the limits of intrinsic repair

Tendon tissue microdamage and the limits of intrinsic repair
Interesting work done in Jess Snedeker's lab was recently published in Matrix Biology. This work evaluated how microdamage to rat tail tendon fascicles influenced the ability of tenocytes (tendon fibroblasts) to aid in repair. This was probed by stretching fascicles up to 8% strain, using CHPs to stain for collagen damage, and then looking at the functional recovery of the fascicles up to 7 days after initial strain. They also looked at cell viability u...

Direct Detection of Collagenous Proteins by Fluorescently Labeled Collagen Mimetic Peptides

Direct Detection of Collagenous Proteins by Fluorescently Labeled Collagen Mimetic Peptides
For those of you interested in how CHP technology works, take a look at this schematic showing how single-stranded CHPs (formerly known as CMPs) are generated either from thermally dissociating the triple helix or by utilizing a photo-sensitive cage group that prevents self-trimerization completely. Once in a single-stranded conformation, CHPs can be used to bind to denatured collagen. This paper goes into detail on how CHPs binding mechanism works and...
Please wait...

Subscribe to our newsletter

Want to be notified when our article is published? Enter your email address and name below to be the first to know!