A great new paper from Dr. Yang Li's group published in ACS NAno utilized in vivo CHPs with a Cy5 fluorescent tag to evaluate collagen destruction in the spine. This in-depth work identified collagen damage in three different animal models, compared the in vivo CHP imaging to micro-CT, histopathology, and performed tissue clearing methods to image using light-sheet fluorescence microscopy to truly identify the anatomic localization of CHP binding in the ...
Another great paper using CHPs! A recent article published in Wound Repair and Regeneration evaluated how the level of collagen denaturation within burned tissues influences the effectiveness of enzymatic debridement (removal of damaged tissue). They showed that low-temperature burns (<65 °C) had inadequate debridement, while high-temperature burns (>65 °C) had very effective debridement. By using CHPs, Sirius red (PSR), and second harmonic gener...
Another great article using CHPs! In this interesting paper, coming out of Dr. Ryan C. Smith's lab from Iowa State, they utilized CHPs to examine the damage done in the midgut of mosquitoes after blood or protein meal feeding. They go on to say that this damage from multiple feedings impacts malaria oocytes' survival. They found that human malaria parasites captured host resources to facilitate their growth after multiple feedings when the basal lamina ...
Interesting work done in Jess Snedeker's lab was recently published in Matrix Biology. This work evaluated how microdamage to rat tail tendon fascicles influenced the ability of tenocytes (tendon fibroblasts) to aid in repair. This was probed by stretching fascicles up to 8% strain, using CHPs to stain for collagen damage, and then looking at the functional recovery of the fascicles up to 7 days after initial strain. They also looked at cell viability u...
For those of you interested in how CHP technology works, take a look at this schematic showing how single-stranded CHPs (formerly known as CMPs) are generated either from thermally dissociating the triple helix or by utilizing a photo-sensitive cage group that prevents self-trimerization completely. Once in a single-stranded conformation, CHPs can be used to bind to denatured collagen. This paper goes into detail on how CHPs binding mechanism works and...
