Scar Formation with Decreased Cardiac Function Following Ischemia/Reperfusion Injury in 1 Month Old Swine

Scar Formation with Decreased Cardiac Function Following Ischemia/Reperfusion Injury in 1 Month Old Swine
An interesting article evaluating the scar formation and cardiac function in pig heart after ischemia/reperfusion (IR) injury. Researchers used CHPs in this study to evaluate the damage immediately following IR and even compared this with Masson's Trichrome staining. They found that immediately after IR, the heart does see a decrease in cardiac function in terms of ejection fraction and fractional shortening but that these conditions did not worsen afte...

The Impact of Skeletal Muscle Contraction on CD146+Lin- Pericytes

The Impact of Skeletal Muscle Contraction on CD146+Lin- Pericytes
Interesting new work published in The American Journal of Physiology- Cell Physiology evaluated the ability of muscle-derived pericyte to contribute to skeletal muscle remodeling in response to training. The researchers used electric stimulation to contract muscles and monitor the response of pericyte cells. They found that the CD146+ Lin- pericyte cells were the most sensitive to contraction and they contribute to upregulating ECM remodeling and angio...

Clumps of Mesenchymal Stem Cell/Extracellular Matrix Complexes Generated with Xeno-Free Conditions Facilitate Bone Regeneration via Direct and Indirect Osteogenesis

Clumps of Mesenchymal Stem Cell/Extracellular Matrix Complexes Generated with Xeno-Free Conditions Facilitate Bone Regeneration via Direct and Indirect Osteogenesis
A recent article published in the International Journal of Molecular Sciences utilized CHPs to verify the health of collagen in their MSC-ECM complexes. The authors developed a clumped-MSC/ECM (C-MSC) complex under serum-free conditions that allows bone regeneration upon implantation without the use of an artificial scaffold. They grew the C-MSC and compared a cellularized vs decellularized C-MSCs for their ability to promote osteogenesis after implanta...

Acoustic modification of collagen hydrogels facilitates cellular remodeling

Acoustic modification of collagen hydrogels facilitates cellular remodeling
A newly published article from Dr. Hocking's Lab used F-CHPs to image collagen remodeling due to acoustic disturbance. They found that exposing the collagen hydrogels to ultrasound waves revealed areas of cell-mediated collagen fiber remodeling. These areas of collagen remodeling were corroborated with SHG imaging. These results indicate that ultrasound exposure does not directly alter triple-helical conformation of collagen, but sensitizes t...

An acute immune response underlies the benefit of cardiac adult stem cell therapy

An acute immune response underlies the benefit of cardiac adult stem cell therapy
EXTRA! EXTRA! Read all about it! New research from Jeffery Molkentin's Lab recently utilized B-CHP to evaluate the mechanical and functional changes in cardiac tissue following an ischemia/reperfusion injury and evaluated the difference in the response following stem cell therapy. In Panel A, the clearly show that there is an increase in ECM remodeling at the infarct border zone compared to an area far away from the infarct. In Panel B, they show that ...

Cell-specific ablation of Hsp47 defines the collagen-producing cells in the injured heart

Cell-specific ablation of Hsp47 defines the collagen-producing cells in the injured heart
Interesting new work from Dr. Jeffrey Molkentin's Lab published in The Journal of Clinical Investigation- Insight, utilized B-CHP to evaluate the effect that Hsp47 deletion has on the collagen production of myocardiocytes (top), endothelial cells (middle), and myofibroblasts (bottom) after transverse aortic constriction (TAC) surgery (a pressure overload model). Their results show that although all three cell types could produce collagen, without Hsp47...

Protection of circadian rhythms by the protein folding chaperone, BiP

Protection of circadian rhythms by the protein folding chaperone, BiP
New work from Dr. Karl Kadler's lab utilized CHPs to see how BiP influences the circadian rhythm of cells. They saw that BiP overexpression resulted in a more robust circadian rhythm but that it led to retention of procollagen in the ER as non-helical collagen and reduced the assembly of collagen fibers. They were able to visualize this by using F-CHPs. Great work and interesting findings! Abstract: ER stress and dysregulation of collagen synthesis are...
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